1 1 10 100 e e e e e e eThe SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 0´ 105 dsD NA (p g/ wel ) R F USpectraMax Glo Steady-Luc 工作液都加 入到微孔板后，震荡摇匀并避光放置 10 min。使用 SpectraMax Glo Steady-Luc Reporter 内置的 SpectraMax Glo Steady- Luc Reporter 实验模板设定程序并通过 SpectraMax i3x 读板机检测发光。检测结 束后，模板会根据数据自动生成一条曲 线。SpectraMax MiniMax 300 细胞成像系统 为 SpectraMax i3x 多功能微孔板检测平台而打造！ • • • • 主要特点 系统全面兼容细胞成像 及微孔板检测技术 独特无标记分析技术无需 对细胞染色即可进行细胞 计数和贴壁度的计算 具有多种检测功能可对 细胞活力及毒性进行全. 8 Antibodies and. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user-configurable. Category 2 and 3 microplate readers use gradient heating as well. Quick imaging and analysis of cells gives you a front-row view of the phenotypic changes that accompany. The NET-specificity of the detected signal was determined by incubation with DNase I (50 U/mL, Qiagen, Germany). Structured Review. Figure 4. 2C). Promega. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesThe SpectraMax i3x reader features a cooled photomultiplier tube (PMT) for low background noise in luminescence. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. The SpectraMax i3x from Molecular Devices is a multi-mode plate reader that can measure spectral-based absorbence and luminescence. A: percent GFP-positive cells calculated using the classification feature in the software. Relative mitochondrial polarization was expressed as the ratio of emission at 590 nm to that at 530 nm (Shin et al. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. Supplier: Molecular Devices. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThe absorbance was recorded on a spectra Max i3x microplate reader (Molecular Devices, USA) at 450 nm. SpectraMax i3x Multi-Mode Microplate Reader SystemSpectraMax® i3x dari Molecular Devices adalah sistem deteksi berbasis multi-mode yang berevolusi menyesuaik. each new lot of plates, to ensure that the center of each well is read. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. It allows you to explore cellular pathways and protein activation and expression in one system. Remove the transport bracket and store the retaining screws in the bag that held the 3. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. It allows you to explore cellular pathways and protein activation and expression in one system. 7. 2. 200-800 nm using Spectra Max i3X microplate reader. 5 decades, with sensitive detection of cells. It allows you to explore cellular . Spectranet customer portalThe SpectraMax Paradigm Multi-Mode Detection Platform is a modular multi-mode microplate detector. Explore a wealth of applications with the future-ready SpectraMax i3x. Page 9 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer Attaching the Imaging Cytometer to the Microplate Reader Attaching the SpectraMax MiniMax Imaging Cytometer to the SpectraMax i3x Instrument requires a work area that is approximately 130 cm (51. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. Besides, it’s possible to examine each page of the guide singly by using the scroll bar. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. 0. . The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Hands-on fun and learning that empowers exploring, inventing and sharing. Reduce background noise The cooled PMT reduces background noise allowing for a more sensitive, wide dynamic range in extremely low light. The SpectraMax i3 is specifically used in this lab for BCA assays and Elisa Assays. It allows you to explore cellular pathways and protein activation and expression in one system. Spectranet customer portal The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. ISM Equipment Classification (Group 1, Class A) This equipment is. SpectraMax i3x读板机针对96孔板和384孔板进行检测时，最低检测下限可至10细胞每孔。 此种检测方法的灵敏度远远高于标准比色法和绝大多数荧光法。 CellTiter-Glo试剂检测线性范围覆盖所有细胞浓度区间，动态学检测范围大于3个数量级且r2 > 0. As. SpectraMax i3x. 3: The cuvette chamber. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax® Microplate Readers and SoftMax® Pro Software. One sensor measures the temperature of the top chamber heating foil, and another is used to measure the bottom chamber heating foil. Multi-Mode Reader. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. SpectraMax i3x plate reader (Molecular Device, contact supplier directly) Vacuum pump system (e. Increased fluorescence performance. The user guide also covers the features and specifications of the SpectraMax MiniMax 300. In this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Itemploysa luciferaselabel(where“label”generallymeansfusedbytheuseofexpressionvectors)forthe oneproteinandaGreenFluorescentProtein(GFP)variantlabelfortheother. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 7°C and the bottom to 36. 06, 0. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Increased fluorescence performance. Microplate assays were performed on a multimode microplate detector (Molecular Devices Spectra Max i3x). Louis, MO) and used as received without further purification. 吸収度マイクロプレートリーダー SpectraMax® Mini. Microplate optimization should be performed the first time the assay is run, and subsequently for . 1. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). 3. The Molecular Devices SpectraMax i3x Microplate Reader is a multi-mode microplate reader, able to measure UV/vis, fluorescence and luminescence. 02, 0. Actual shake speed is based on the microplate format. ZERO BIAS - scores, article reviews, protocol conditions and more. SoftMax Pro GxP Compliance Software. Use the 3. 314 low lamp level warning. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Description 1. SpectraMax i3Instrumenttovisuallyinspectyoursampleandtoruncell-basedassaysat cellularorwhole-cellresolution. The SoftMax Pro Software quickly. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. 価格、見積もり、お近くの販売店を. SpectraMax Microplate Readers. XPS spectra were obtained from PHI Versaprobe II. Capture Flash Assays with Ease. CRITICAL STEP Avoid air bubbles; reverse pipetting the luciferase reagent into the plate is a good way to achieve this. Fluorescence emission spectra were collected on an ELISA molecular system (Spectra Max i3x). The intensity of the emitted light can be measured easily with the SpectraMax i3x reader. This way you’ll save time on finding the necessary info. 04, 0. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013. It also integrates with the. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. It allows you to explore cellular pathways and protein activation and expression in one system. Enzyme activity was presented as absolute activity in a unit of nmol g −1 dry soil h −1. Published Dec 16, 2020 | Updated Dec 16, 2020. See5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideMolecular Interactions. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellSpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. Detection of pro-inflammatory factors. The LDH activity in the cultural media was measured based on the reaction between LDH and lactic acid, which led to the generation of pyruvic acid, and pyruvic acid could become brown in alkaline environments with 2,4. The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely. Carbonyl assay The plates for carbonyl detection were prepared as described. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Patented user-exchangeable cartridge design expands the system's detection capability making it. Analysis. Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. About the reader setup recommendations The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. No Stain Analysis: Eliminate cell staining for cell counting and confluence measurements using. 2 (Molecular Devices, San Jose, CA, USA). The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. applications, including dual luciferase and ATP assays. 4. The plate was covered and incubated for one hour at room temperature. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles). TP medium was used as a blank and its fluorescence intensity was subtracted from that of mCherry or from chlorophyll auto-fluorescence intensity. 0 | 9 reviews | Write your own review Measures Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgradesMolecular Devices SpectraMax i3x Multi-Mode Microplate Platform. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. 발광 (Luminescence) 리더기. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. As shown in the results above, the DELFIA cell proliferation assay combined with the SpectraMax i3 reader gives researchers a very sensitive test for measuring cell proliferation. 314 low lamp level warning. Align the two slots on the bottle holder with the two pegs on the rail. Fourier transform infrared (FT-IR) spectra of cCQD were obtained on a. 0 mm hex key to remove the six blue retaining screws that secure the transport bracket (B) to the instrument. The SPECTRAmax 340PC can mea- Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. 7 mm for all shake intensities. Downloads. , 2011). The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. ZERO BIAS - scores, article reviews, protocol conditions and moreWe would like to show you a description here but the site won’t allow us. ) wide and 65 cm (25. Introduction. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. All test measurements and calculations are handlediD5. The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. Description 1. SCGPU05RE)A SpectraMax ® i3x was used to determine uo-rescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Thetypeof. flValitaTiter (i3x i3) ValitaTiter (iD5) ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. The standard solutions (0. 0´ 104 4. Enzyme activity was presented as absolute activity in a unit of nmol g −1 dry soil h −1. Price from $9. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. The MiniMax cytometer with SoftMax® Pro Software can be used to image and analyze a variety of cells. Capture flash assays with ease The SpectraMax i3x Injector Cartridge with SmartInject® Technology expands your research capabilities to include flash-based applications including dual luciferase and ATP assays. Find regional support. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. comThe SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™. Explore a wealth of applications with the future-ready SpectraMax i3x. 在SpectraMax i3x和SpectraMax iD5微孔板读板机上使用 SoftMax Pro软件绘制标准曲线，并进行4参数曲线拟合(图 4)，结果见表2。使用EC50评估该测定方法的适用性，EC50值 应低于150 nM。对于两个读板机，EC 50值均低于85 nM，处 于可接受范围内。Cisbio将可接受的信噪比定义为≥. SeeSpectraMax MiniMaxImagingCytometeronpage85. In this product video, see how the SpectraMax M5 quickly reads samples. How to properly unlock iD series instrument. Ease of use. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Also refer to this Multimode Reader Comparison Chart. SpectraMaxi3Multi. View online or download 1 Manuals for Molecular Devices SpectraMax i3x. Average 98 stars, based on 1 article reviews. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Patented user-exchangeable cartridge design expands the system's detection capability making it. 3 In this application note we demonstrate a linear dynamic range of 6 decades for recombinant firefly and Renilla luciferases, as well as linear detection of luciferase-transfected. 22 μ m fi ltration unit (Sigma, cat. 2. 1 mg/mL density of resazurin, protected of the light, incubated for 1 h at room temperature. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . The SpectraMax® i3x Multi-Mode Microplate Reader with the SpectraMax® MiniMax™ 300 Imaging Cytometer is equipped with transmitted light (TL), green fluorescence (Ex/Em: 460/541), and red (625/713) fluorescence channels. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. The Molecular Devices SpectraMax i3x is a multi-mode microplate reader, able to measure UV/vis, fluorescence and luminescence. Reduce background noise The cooled PMT reduces background noise allowing for a more sensitive, wide dynamic range in extremely low light. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. techsupport. We would like to show you a description here but the site won’t allow us. The corrected absorbance is within 5% of the value. The resulting absorbance was measured at 490 nm, Spectra Max i3x (Molecular Devices, USA). The chemical reduction of resazurin was evaluated incubating each well of the microtiter plate with 10 μL (10% of final well volume) of 0. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium. 3) At the bottom of the strip, click on "Show. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. 312 gain calibration failed. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. Table 1. Italic and boldface type are used for emphasis. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Thetypeof luciferase(Renilla. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Spectramax I3x, supplied by Danaher Inc, used in various techniques. Select PMT gain o PMT gain = energy used by detector to collect signal o Low gain for samples with high expected signalSpectraMax i3x Multi-Mode Microplate Reader. ) wide and 65 cm (25. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. Also refer to this Multimode Reader Comparison Chart. Quantification of NETs was performed with the SpectraMax i3x microplate-reader (Molecular Devices, United Kingdom). 6 in. Item SpectraMax i3x Multi-Mode Microplate Readers; Company Molecular Devices LLC; Catalog Number SpectraMax i3x <Disabled> This product is no longer available on Biocompare. 3: The cuvette chamber. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Immunofluorescence of In Vitro LPS-Stimulated Neutrophils. i3. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. 19 Switch on the SpectraMax i3x plate reader and launch SoftMax Pro software. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. See SpectraMax® Microplate Reader User Manual Download Page. Myeloperoxidase (MPO) Assay. Supplier: Molecular Devices. 0´ 104 8. Then, the cell area was calculated for each. 모든 제품. 0. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Molecular Devices Software. Molecular Devices’ ScanLater Western Blot Detection Cartridge uses TRF detection to turn its SpectraMax i3X and Paradigm plate readers into Western blot readers. ATP (“flash” luminescence using . If you do not have an iLab account you must first make one: SpectraMax iD3 User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax iD3 Multi-Mode Microplate Reader, a versatile instrument that measures absorbance, fluorescence, and luminescence. We would like to show you a description here but the site won’t allow us. SpectraMax M5. Cell counters for accurate and. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. Unpacking and setting up the instrument includes the following procedures: • Unpacking the instrument and saving the original packaging. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Description 1. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. SpectraMax i3x, onboard luminescence 121. no. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. 58% for the detection module and 2. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Sunnyvale, CA, USA) with excitation at 475 nm. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Methods. Spectramax I3x, supplied by Danaher Inc, used in various techniques. Figure 4. Animal imaging assay was performed on a IVIS R Lumina Series III small animal optical system. This application note describes the use of the SPECTRAmax ® GEMINI XS from Molecular Devices to study the performance of the LIVE/DEAD ® Viability/Cytoxicity Assay Kit for animal cells. Transfected cells were imaged on the SpectraMax MiniMax 300 Imaging Cytometer. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Briefly, after exposed to ZnO/GO and Cd, cells were collected and washed with ice-cold PBS twice and then re-suspended in 200 μL binding buffer. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Microplate reader assays requiring infrared (IR) or red-shifted. Table 1. To read HTRF®, the SpectraMax i3x® must be first equipped with the SpectraMax i3x® Cisbio HTRF® cartridge, which enables the simultaneous measurement of both 620nm donor and 520 nm acceptor emissions. Synthesis of NORP Synthesis of compound 1. We would like to show you a description here but the site won’t allow us. Settings not required by a reader are indicated by ‘---’. Flow cytometry was used to measure ROS concentrations, based on fluorescence intensity (FL-1, 530 nm) of 10,000 cells. 01 0. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. Measured using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. Bioz Stars score: 98/100, based on 1 PubMed citations. The SpectraMax® i3 Multi-Mode Microplate Reader Detection Platform evolves with your future research needs and offers a breadth of application possibilities. Apoptosis was measured by the Annexin V-FITC/PI Apoptosis Detection Kit. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Microplate Reader. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax 190 control panel are shown in boxed Helvetica type. After incubation at 37 °C and 5% CO 2 for 20 min, fluorescence images were taken using Spectra Max i3x with MiniMax 300 Imaging Cytometer (Molecular Devices, San Jose, CA) using the setup of excitation and emission provided in the SoftMax Pro 7. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects and changing objectives, the i Series has you covered. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. 305 reference level saturation. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. ; The top 3 importers of Spectramax are India with 365. 2. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. 306 plate air cal fail,low light. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. SpectraMax® iD3/iD5. SpectraMax i3x Multi-Mode Microplate Reader; SpectraMax MiniMax 300 Imaging Cytometer *Here, the NucView 488 with DMSO formulation was used. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Red and green fluorescence was then measured (emission at 590 and 530 nm, respectively, with excitation at 490 nm) using a fluorescence microplate reader (Spectra Max i3x, Molecular Devices Inc. After incubation at 37 °C and 5% CO 2 for 20 min, fluorescence images were taken using a Spectra Max i3x with MiniMax 300 Imaging Cytometer (Molecular Devices, San Jose, CA, USA) with the excitation and emission setup provided with SoftMax Pro 7. Native or oxidized AN (40 μM) and FN (0. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. The SpectraMax i2X is used for cell migration, cell. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. 1. Increased fluorescence performance. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes This assay workflow is easily performed using the SpectraMax i3x Multi-Mode Microplate Reader with SpectraMax Injector Cartridge, which is fully DL Ready validated. The calculated LOQ values were 0. 4 / 5. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. at 540 nm using a Spectra Max i3x microplate reader (Molecular devices, San Jose, CA). SpectraMax i3x 一套系统即可以帮助你通过科学手段解开细胞通路的奥秘，也可以检测并分析出 样品中蛋白质的活性和表达情况。SpectraMax i3x 检测系统可以根据未来实验需求，无需售后工 程师协助即可加入全新检测功能模块，真正做到一次投入终身受益。1) Select the correct plate and the wells that you want to measure. The absorbance at 450 nm was read with a Spectra Max i3X microplate spectrophotometer (Molecular Devices, Sunnyvale, CA, USA), and the data at 30–40 min post-treatment were analyzed. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. 在SpectraMax i3x和SpectraMax iD5微孔板读板机上使用 SoftMax Pro软件绘制标准曲线，并进行4参数曲线拟合(图 4)，结果见表2。使用EC50评估该测定方法的适用性，EC50值 应低于150 nM。对于两个读板机，EC 50值均低于85 nM，处 于可接受范围内。Cisbio将可接受的信噪比定义为≥. each new assay. For more detailed information and technical support of Molecular Devices instruments or software,The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. Quick imaging and analysis of cells gives you a front-row view of the phenotypic changes that accompany. The user guide also covers the features, specifications, and software of the reader, as well as the installation and. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. com. Research Article Streptococcus lutetiensis Induces Autophagy via Oxidative Stress in Bovine Mammary Epithelial Cells Peng Chen ,1 Jingyue Yang,1 Naiwen Wu,1 Bo Han,1 John P. View online or download 1 Manuals for Molecular Devices SpectraMax i3x. 304 signal level saturation. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . 303 unable to cal dark current. QBT Fatty Acid Transporter Assay. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology*On the SpectraMax i3x reader only. Bound crystal violet was dissolved with 10% acetic acid and the optical density (OD) at 570 nm was determined using a Spectra Max i3X spectrophotometer (Molecular Devices). The SpectraMax i3x’s user-upgradeable application modules will future-proof your research to include most assay capabilities of the SpectraMax iD3 and iD5 readers, as well as additional capabilities such as imaging. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . Promega. SoftMax Pro. com. HTRF IP standard curve. Recommendations for SpectraMax i3/i3x plate readers The Molecular Devices SpectraMax i3/i3x allows use of the pH-Xtra Glycolysis Assay with time-resolved fluorescence (TRF) detection. Pick the best speed, sensitivity, and price for your needs. SpectraMax® i3x Multi-Mode Microplate Reader with MiniMax™ imaging cytometer Integrated multi-mode microplate reader and imaging system • Ideal for cell counting, label-free imaging, and cell proliferation • Western blot, imaging, and injectors on one configurable microplate reader • Stain free, automated cell countingusing the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. The SpectraMax® i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6-, 384- well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. 2 only) SpectraMax M-series Microplate readers. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Example: [Drawer]. spectramax i3x (Danaher Inc)The fluorescence emission intensity spectra were recorded using a microplate reader (Spectra Max® i3x, Molecular Devices, LLC. The FRET ratio is defined as the relative fluorescence intensity of an acceptor normalized to the total fluorescence intensity. Bioz Stars score: 98/100, based on 1 PubMed citations. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. Enzyme Activation Analysis;. 2. 蛍光 発光 ELISA. ValitaTiter Plus settingsThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Image analysis provides accurate data. 316 grating motor driver faulty. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a. Meanwhile, the samples were detected with a fluorometric reader Spectra Max i3x (MD, America) at 488 nm excitation wavelength and 525 nm emission wavelength. Find regional support. 2. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataThe SpectraMax i3x reader and MiniMax cytometer can be used with FluoroBlok cell culture inserts to directly count cells from high-quality images to measure cellular migration over time. spectramax i3x (Danaher Inc)The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. 11. 10, and 0. 58% for the detection module and 2. Videos & Webinars SpectraMax i3x - Detection Module ChangeSpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. The protocols are also available on the SoftMax Pro Software protocol sharing. 800. Contact Us Phone: +1.